4/19/2023 0 Comments Fityk raman deconvolution![]() These kinds of premises have become the driving force to develop stem cell therapy in otolaryngology. Research is being carried out on the development of a biological method to repair a damaged cochlea that can restore normal hearing without any implant materials or hearing aid devices. There are many therapeutic solutions to treat this disease, such as hearing aids and cochlear implants, that can provide good retrieval of the hearing function. Hearing loss is a common human disease caused by irreversible damage to hair cells and spiral ganglion neurons in the mammalian cochlea. Otolaryngology, like cardiac surgery or neurosurgery, is looking for new solutions in the field of therapy methods using electric conductive nanomaterials for the construction of both implantable electrodes and nanomaterials allowing the construction of substrates for tissue engineering and stem cell therapy. All these elements, used to regulate processes in stem cells, are very important factors creating favorable conditions in stem cell therapy. Interactions between stem cells and their environment in vivo conditions are very complex involving biochemical factors, extracellular matrix components, and physical factors affecting cell behavior. Such tissue supports are attractive solutions for the needs in stem cell therapy. It was shown that introducing electric field stimulation in cell-based treatment is a beneficial physical factor affecting the effectiveness of tissue engineering methods. Research is being conducted on the development of alternative cellular activation processes, and electrical stimulation is one of the directions of research in nerve engineering, as well as in the treatment of cardiac and skeletal muscles. It is well known that endogenous electric fields play an important role in controlling cellular functions, such as morphology, gene expression, proliferation, and migration. Cells colonizing the tissue scaffold have desirable conditions for proliferation and differentiation. Tissue engineering is an area that is based on biomimetic scaffolds modified with bioactive agents. The development of regenerative medicine and tissue engineering is to a large extent related to the achievements of nanotechnology. Research on new forms of nanomaterials has resulted in the development of a number of new solutions in the field of medical therapies and diagnostics, including biosensors, implantable electrodes, materials for drug carriers and anticancer therapy, and development of new methods for tissue engineering and regenerative medicine. Materials with reduced dimensions to nanoscale, i.e., nanomaterials, are often characterized by specific physical and chemical properties, which are of particular interest in terms of potential medical applications. Modern medicine applies more and more therapeutic solutions based on the achievements of nanotechnology and nanomaterials. The ECNFV introduced into cell culture did not affect the repair processes in the cells contacting them. The ECNFV nanofibers were not cytotoxic, whereas ECNF nanofibers contacted with both types of cells indicated a cytotoxic effect. Fibroblasts contacted with the as-received carbon nanofibers (ECNF) showed a significantly higher level of DNA damage compared to control and oxidized carbon nanofibers (ECNFV). Genotoxicity study conducted by means of comet assays revealed significant differences between both carbon nanofibers. Biological tests (genotoxicity, fibroblast, and human osteoblast-like MG63 cultures) were carried out in contact with both materials. The oxidative treatment of carbon nanofibers significantly changed their surface morphology and physical properties (wettability, surface electrical resistance). The morphology, microstructure, and surface properties of both materials were analyzed. Both types of carbon fibrous mats were studied using scanning electron microscopy (SEM), high-resolution transmission electron microscopy (TEM), XRD, and Raman spectroscopy. The oxidative treatment led to partial removal of a structurally less-ordered carbon phase from the near-surface region of the carbon nanofibers. Carbon nanofibers obtained by carbonization of the PAN nanofibers to 1000☌ (electrospun carbon nanofibers (ECNF)) were additionally oxidized in air at 800☌ under reduced pressure (electrospun carbon nanofibers oxidized under reduced pressure (ECNFV )). The aim of this work was to manufacture, using the electrospinning technique, polyacrylonitrile- (PAN-) based carbon nanofibers in the form of mats for biomedical applications. ![]()
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